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Let's Talk Micro

Luis Plaza
Let's Talk Micro
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  • 201: Spotlight: Rethinking AST-Disk Diffusion on Early Growth
    Last week, we discussed early growth isolates for antimicrobial susceptibility testing (AST) and the importance of obtaining results sooner. This week, we revisit an earlier study that looked at early growth but using disk diffusion. In this episode, I was joined by Dr. Carey-Ann Burnham, Dr. Daniel Webber, and Meghan Wallace to discuss their work. Tune in to hear how this approach could help deliver AST results faster, the challenges of adopting early growth methods, and what it means for clinical microbiology labs. This episode was originally released in 2022 (Episode 39: “Talking disk diffusion performed on early growth”).   Link to disk diffusion study: https://doi.org/10.1128/jcm.03007-20   Check out the website: https://www.letstalkmicro.com/ Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  
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  • 200: Early Growth, Faster Answers: Rethinking AST
    In this episode of Let’s Talk Micro, Dr. Jennifer Dien Bard and Dr. Lucas Osborn discuss their recent study on early growth antimicrobial susceptibility testing (AST). Traditional AST often requires 18–24 hours of incubation of isolates, delaying targeted treatment. Their research shows that using 6-hour early growth isolates with both automated and manual methods delivers accurate results up to 18 hours faster—without added costs or specialized equipment. The conversation explores the study design, agreement rates with standard methods, and what faster AST means for patient care and antimicrobial stewardship.  Link to study: https://doi.org/10.1128/jcm.00236-25 Link to disk diffusion study: https://doi.org/10.1128/jcm.03007-20   Check out the website: https://www.letstalkmicro.com/ Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  
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  • 199: Antibiotics: What’s New in Treatment
    This week, Luis is joined by Dr. Ashlan Kunz-Coyne to discuss some of the newest antibiotics for urinary tract infections (UTIs). They explore why extended-spectrum beta-lactamases (ESBLs) are a growing concern, the impact of E. coli sequence type 131, and where new agents like cefepime–enmetazobactam, sulopenem, and tebipenem fit into treatment. The conversation also highlights clinical data, stewardship considerations, and how the infectious diseases and microbiology communities can work together to preserve these options. Plus, Dr. Kunz-Coyne reveals her favorite bug—Stenotrophomonas maltophilia—and why it’s unlike any other.    Link to sulopenem episode: https://directory.libsyn.com/episode/index/id/35868135   Check out the website: https://www.letstalkmicro.com/ Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  
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  • 198: Let’s Talk: The Evolution of AST
    Season 5 premieres today!  Antimicrobial susceptibility testing (AST) is essential for guiding effective treatment—but how has it evolved, and what challenges are we still facing? In the first episode of this series in collaboration with bioMérieux, Dr. Andrea Prinzi joins Let’s Talk Micro to explore the evolution of AST—from early methods to modern platforms like VITEK 2. We discuss the known issue of outdated breakpoints in clinical practice, the challenges with regulatory hurdles, and the impact of the 21st Century Cures Act. What is the Cures Act, and how does it affect the way breakpoints are adopted? Why are delays still happening? And most importantly—what can laboratories, organizations, and manufacturers do to drive change? Tune in to understand where we’ve been, where we are, and where we need to go in the world of AST.   This episode is sponsored by bioMérieux, a global innovator in infectious disease diagnostics.   Resources from bioMérieux: https://info.biomerieux.com/clin_us_2024_ams_handbook?utm_source=podcast&utm_medium=show-notes&utm_campaign=LetsTalkMicro Additional breakpoint links: https://doi.org/10.1128/jcm.00106-25   Check out the website: https://www.letstalkmicro.com/ Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro
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  • TBT: Talking Breakpoints with Dr. Andrea Prinzi
    This episode of Let’s Talk Micro features Dr. Andrea Prinzi, a clinical microbiologist and frequent contributor to the American Society for Microbiology. Andrea joins the podcast to discuss a critical topic in clinical microbiology: antimicrobial susceptibility testing and breakpoints. Are labs using outdated breakpoints—and what does that mean for patient care? How are breakpoints determined and updated? Dr. Prinzi breaks it all down and shares insights from her article on the subject. Tune in to learn why staying current on breakpoints matters and what steps labs can take to improve antimicrobial stewardship. This episode was originally published on May 5th, 2022.   Check out the website: https://www.letstalkmicro.com/ Link to articles: https://asm.org/Articles/2022/February/Updating-Breakpoints-in-Antimicrobial-Susceptibili https://doi.org/10.1128/jcm.00106-25   Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  
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Clinical microbiology explained in simple terms, suitable for students and microbiologists alike. We discuss organisms, reactions, infectious diseases, and more. Hosted by Luis Plaza, a Medical Laboratory Scientist and microbiologist, who also shares his experiences in the field.
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