Inside Immunoassays: Strategies to Maximize Your Immunoassay Data and Insights
Immunoassays are pivotal in scientific research, enabling the precise identification and measurement of specific analytes. They pave the way for investigations into disease mechanisms, discovering biomarkers, and evaluating the efficacy of potential therapeutic interventions.The key reagents included in an immunoassay can significantly impact reliability and reproducibility, underscoring the importance of understanding critical components within immunoassay kits.When selecting an immunoassay platform, it's essential to consider critical elements such as multiplexing capabilities and sensitivity needs as part of the experimental design.Additionally, understanding the various assay formats and their data outputs can aid in the immunoassay selection process.In this episode, immunoassay experts give practical guidance in these areas, empowering researchers to achieve more precise and meaningful results, thereby advancing knowledge and understanding in various scientific fields.MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany.For Research Use Only. Not For Use In Diagnostic Procedures.
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AI-Powered 3D Organoid Analysis
Multicolor 3D fluorescent confocal microscopy presents significant challenges in image acquisition and data analysis. However, AI is making a massive impact on 3D spatial biology. This episode of Listen In explores the incorporation of AIVIA, an AI-powered image analysis software, into workflows to address these challenges in analyzing multicolor fluorescent microscopy data of 3D organoids. See how AIVIA’s tools enhance the visualization and quantification of cellular structures and organoids, enabling precise segmentation and analysis of fluorescent signals. And get a showcase of how AIVIA supports Python-based image analysis for microscopists. Watch the full presentation here: https://events.bitesizebio.com/ai-powered-3d-organoid-analysisBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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High-Throughput and Wide-Range Protein Concentration Determination of Monoclonal Antibodies
In this episode of Listen In, explore high-throughput and wide-range protein concentration analysis that can deliver a 20-fold improvement in time and budget savings.Protein concentration is an obligatory critical quality attribute necessary to determine total protein content in biopharmaceutical formulations. Concentration determination is necessary before many downstream assays. A UV-based spectrophotometric assay using the protein’s extinction coefficient is the most typical method. Conventional UV methods or a specialized approach using variable pathlength slope spectroscopy could take one day to measure 15 samples in triplicate. Larger batches would require multiple analysts for a single-day turnaround time. With vast studies spanning multiple projects, protein concentration analysis becomes a key deliverable before all other methods can be performed. Thus, efficient rapid concentration analysis with a minimal volume requirement is essential. Hit play to learn how the Lunatic (Unchained Labs, Pleasanton, CA) offers rapid, plate-based concentration measurement with minimal prep and sample volume requirements. It utilizes only two microliters per sample, can analyze 96 wells in less than 12 minutes, and provides accurate measurements over a broad concentration range, from 0.03 mg/mL to 176 mg/mL.Watch the full presentation here: https://events.bitesizebio.com/high-throughput-and-wide-range/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Multiplexing Magic: How MULTI-seq Revolutionizes Single-Cell RNA Sequencing
Learn more about the capabilities of MULTI-seq in single-cell sequencing in this episode of Listen In. Developed by the Gartner Lab at UCSF, this innovative multiplexing method increases sample and cell throughput while minimizing cost and improving your data quality. From modest experiments to expansive 96-plex studies and beyond, MULTI-seq enables truly mechanistic studies. Hit play to explore the potential of this tool and how it could benefit your research.Watch the full presentation here: https://events.bitesizebio.com/multiplexing-magic-how-multi-seq/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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Multi-View Light Sheet Microscopy to Track Cell Lineages in Tissue Morphogenesis
A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time. Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy. This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics. In this episode of Listen In, hear how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity. See two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development.We also explore the technology behind the Leica Viventis LS2 Live, which combines dual-view illumination and detection in an open-top configuration. You will also learn how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems. Watch the full presentation here: https://microscopyfocus.com/multi-view-light-sheet-microscopy-to-track-cell-lineages-in-tissue-morphogenesis/"Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever.
Each episode is an opportunity to gain the valuable insights you need to advance your research.
From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results.
Tap into the experience and expertise of leading researchers and commercial specialists to drive your research projects forward efficiently and productively. Listen In now!
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