Detecting Rare T-Cell Responses at Scale with ELISpot and FluoroSpot
Detecting rare antigen-specific T-cell responses requires assays that are both sensitive and scalable. Traditional methods like flow cytometry often struggle with high-throughput needs or with preserving precious sample material.In this episode of Listen In, you’ll discover how ELISpot and FluoroSpot assays overcome these challenges. These cell-based immunoassays measure cytokine and antibody secretion at the single-cell level, offering a sensitive, robust, and accessible way to capture rare immune responses.You’ll learn how ELISpot and FluoroSpot can be applied in vaccine studies, biotherapeutic evaluation, and cell and gene therapy development. The episode also explores practical guidance on assay setup and execution, the advantages of multiplex cytokine detection with FluoroSpot, and how these platforms can be qualified for use in preclinical and clinical trials.With their unique combination of sensitivity and ease of use, ELISpot and FluoroSpot provide a valuable alternative to flow cytometry, especially when high throughput and efficient data collection are priorities.Watch the full presentation here: https://events.bitesizebio.com/detecting-rare-t-cell-responses-atBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
--------
1:04:09
--------
1:04:09
From Surplus to Savings: Tackling Unused Lab Products in Life Sciences
In the life sciences, an astounding amount of unused products and materials end up in landfills instead of contributing to research progress. This issue arises across the product lifecycle, from liquidated biotech start-ups that abandon equipment to over-purchasing and pipeline deprioritization at successful firms. Even well-intentioned suppliers encounter forecasting challenges that leave perfectly good items discarded!In this episode of Listen In, explore the root causes of waste in our industry, as we draw on real-world examples of failed companies, discontinued pipelines, and canceled orders. We will examine the ripple effects this has on both operational efficiency and the broader sustainability goals of life science organizations. We will also introduce Wasteless Bio, a dedicated marketplace designed to keep high-quality, unused products in circulation—helping researchers access discounted reagents and instruments while reducing environmental impact. Whether you are a biotech executive, lab manager, or supplier, you will gain a clearer understanding of how to rescue valuable resources from the trash and reinvest them back into scientific innovation.Watch the full presentation here:Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
--------
30:44
--------
30:44
Automated Hepatocyte Counting: New Technology for Simple, Reliable Counts
Automated counting of certain cell types is notoriously challenging due to their irregular shapes, variable nuclei number, autofluorescence, and often high amounts of debris in samples.However, with the latest developments in cell-counting hardware and app technology, automated counting of even challenging cells is simpler than ever.In this episode, see how you can achieve reliable automated cell counts with the CellDrop Automated Cell Counter. Using hepatocytes as an example, you will learn how the machine learning technology integrated into DeNovix’s Hepatocyte app can save time, reduce human error, and provide consistent results.Watch the full presentation here: https://events.bitesizebio.com/automated-hepatocyte-counting-newBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
--------
30:07
--------
30:07
Pioneering Biomarker Discovery In Mucinomics Through Mass Spectrometry
In this episode of Listen In, we showcase cutting-edge mass spectrometric analysis of mucins, including the characterization of mucinases, enrichment techniques, and complete mucinomic mapping of translationally relevant mucin proteins.Mucins are a diverse family of high molecular weight glycoproteins that play critical roles in protecting and lubricating epithelial surfaces, making them integral to numerous physiological processes and disease states. As a promising class of biomarkers, mucins may offer unique insights into various pathologies, such as cancer and chronic inflammatory diseases. However, their complex structures and extensive glycosylation present significant challenges in their study and application.Mucin-domain glycoproteins are densely O-glycosylated and play key roles in many biological functions. However, their dense O-glycosylation remains enigmatic both in the glycoproteomic landscape and structural dynamics, primarily due to the challenges associated with studying mucin domains. In this episode, you will get an introduction to the (glyco)proteomic techniques used in Mucinomics and learn how proper sample preparation of mucin-containing tissues for mass spectrometry is key to discovery.MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany.Watch the full presentation here: Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
--------
1:02:40
--------
1:02:40
How to Multiplex Any Assay: User Experience of the xMAP INTELLIFLEX®
In this episode, learn about latest advancements in multiplex assay technology.Hear from real user experience how the advanced xMAP INTELLIFLEX® DR-SE Instrument revolutionizes assay multiplexing, enabling simultaneous rapid data acquisition for multiple sample parameters to expand the depth and breadth of your research.Watch the full presentation here: https://events.bitesizebio.com/user-experience-of-the-xmapThis compact, flow-based multiplex platform integrates xMAP® Technology with modern enhancements to streamline assay development, enhance user experience, and deliver fast, reliable results by acquiring data for two parameters per analyte simultaneously.Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever.
Each episode is an opportunity to gain the valuable insights you need to advance your research.
From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results.
Tap into the experience and expertise of leading researchers and commercial specialists to drive your research projects forward efficiently and productively. Listen In now!
https://bitesizebio.com/listen-in/
Österreich